Galectins-3 and-7, but not galectin-1, play a role in re-epithelialization of wounds

被引:157
作者
Cao, ZY
Said, N
Amin, S
Wu, HK
Bruce, A
Garate, M
Hsu, DK
Kuwabara, I
Liu, FT
Panjwani, N
机构
[1] Tufts Univ, Sch Med, Dept Ophthalmol, Ctr Vis Res, Boston, MA 02111 USA
[2] Tufts Univ, Sch Med, New England Eye Ctr, Boston, MA 02111 USA
[3] Tufts Univ, Sch Med, Dept Biochem, Boston, MA 02111 USA
[4] Univ Calif Davis, Sch Med, Dept Dermatol, Davis, CA 95616 USA
关键词
D O I
10.1074/jbc.M200981200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Disorders of wound healing characterized by impaired or delayed re-epithelialization are a serious medical problem. These conditions affect many tissues, are painful, and are difficult to treat. In this study, using cornea as a model, we demonstrate for the first time the importance of carbohydrate-binding proteins galectins-3 and -7 in re-epithelialization of wounds. In two different models of corneal wound healing, re-epithelialization of wounds was significantly slower in galectin-3-deficient (gal3(-/-)) mice compared with wild-type (gal3(+/+)) mice. In contrast, there was no difference in corneal epithelial wound closure rates between galectin-1-deficient and wild-type mice. Quantitation of the bromodeoxyuridine-labeled cells in gal3(+/+) and gal3(-/-) corneas revealed that corneal epithelial cell proliferation rate is not perturbed in gal3(-/-) corneas. Exogenous galectin-3 accelerated re-epithelialization of wounds in gal3(+/+) mice but, surprisingly, not in the gal3(-/-) mice. Gene expression analysis using cDNA microarrays revealed that healing corneas of gal3(-/-) mice contain markedly reduced levels of galectin-7 compared with those of gal3(+/+) mice. More importantly, unlike galectin-3, galectin-7 accelerated re-epithelialization of wounds in both gal3(-/-) and gal3(+/+) mice. In corresponding experiments, recombinant galectin-1 did not stimulate the corneal epithelial wound closure rate. The extent of acceleration of re-epithelialization of wounds with both galectin-3 and galectin-7 was greater than that observed in most of the published studies using growth factors. These findings have broad implications for developing novel therapeutic strategies for treating nonhealing wounds.
引用
收藏
页码:42299 / 42305
页数:7
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