Isolated microparticles, but not whole plasma, from women with preeclampsia impair endothelium-dependent relaxation in isolated myometrial arteries from healthy pregnant women

被引:101
作者
VanWijk, MJ
Svedas, E
Boer, K
Nieuwland, R
VanBavel, E
Kublickiene, KR
机构
[1] Univ Amsterdam, Acad Med Ctr, Dept Obstet & Gynecol, NL-1100 DD Amsterdam, Netherlands
[2] Univ Amsterdam, Acad Med Ctr, Dept Phys Med, NL-1100 DD Amsterdam, Netherlands
[3] Univ Amsterdam, Acad Med Ctr, Dept Clin Chem, NL-1100 DD Amsterdam, Netherlands
[4] Huddinge Univ Hosp, Karolinska Inst, Dept Obstet & Gynecol, S-14186 Huddinge, Sweden
关键词
isolated myometrial arteries; preeclampsia; plasma; microparticles; endothelial function;
D O I
10.1067/mob.2002.127905
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: This study was performed to establish whether microparticles from plasma of women with preeclampsia cause endothelial dysfunction, as described for isolated myometrial arteries in preeclampsia. STUDY DESIGN: Myometrial arteries were isolated from biopsy specimens obtained at cesarean delivery from healthy pregnant women (n = 22) and mounted in a wire myograph. Bradykinin concentration-response curves were obtained before and after 1-hour incubation or after overnight incubation with one of the following preparations of plasma from individual women with preeclampsia (n = 16): whole plasma, microparticle-free plasma, isolated microparticles resuspended in physiologic saline solution or physiologic saline solution. Overnight incubation was also performed with microparticles isolated from healthy pregnant women (n = 6). One-hour incubation was performed with 2% or 10% solution and overnight incubation with 5% solution. RESULTS: No effect of preeclamptic plasma, with or without microparticles, on bradykinin-mediated relaxation was observed. Overnight, but not 1-hour, incubation with preeclamptic microparticles caused abolishment of bradykinin-mediated relaxation in contrast to healthy pregnant microparticles (P < .005). CONCLUSION: Preeclamptic microparticles, but not healthy pregnant microparticles cause endothelial dysfunction in isolated myometrial arteries from healthy pregnant women after overnight incubation, whereas other preeclamptic plasma constituents protect the endothelium from this effect.
引用
收藏
页码:1686 / 1693
页数:8
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