Mitochondrial DNA content of human spermatozoa

被引：74

作者：

Díez-Sánchez, C ^{[1
]}

Ruiz-Pesini, E ^{[1
]}

Lapeña, AC ^{[1
]}

Montoya, J ^{[1
]}

Pérez-Martos, A ^{[1
]}

Enríquez, JA ^{[1
]}

López-Pérez, MJ ^{[1
]}

机构：

[1] Univ Zaragoza, Dept Bioquim & Biol Mol & Celular, Zaragoza 50013, Spain

来源：

BIOLOGY OF REPRODUCTION | 2003年 / 68卷 / 01期

关键词：

gamete biology; sperm; sperm maturation; spermatogenesis;

D O I：

10.1095/biolreprod.102.005140

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sperm mitochondria play an important role in spermatozoa because of the high ATP demand of these cells. Different mitochondrial DNA (mtDNA) mutations and haplogroups influence sperm function. The mtDNA dose also contributes to genetic variability and pathology in different tissues and organs, but nothing is known about its relevance in the performance of spermatozoa. We estimated the variability in mtDNA content within a population of men. Different mtDNA:nuclear DNA ratios were characteristic of progressive and nonprogressive spermatozoa, confirming the influence of mtDNA content on sperm functionality. We also estimated that the absolute content of mtDNA was 700 and 1200 mtDNA copies per cell in progressive and nonprogressive human spermatozoa, respectively. These results suggest that a marked increase of mtDNA copy number per cell volume takes place during spermatogenesis.

引用

页码：180 / 185

页数：6

共 45 条

[1] Leigh syndrome in an infant resulting from mitochondrial DNA depletion [J].

Absalon, MJ ;

Harding, CO ;

Fain, DR ;

Li, L ;

Mack, KJ .

PEDIATRIC NEUROLOGY, 2001, 24 (01) :60-63

[2]

AITKEN RJ, 1984, J ANDROL, V5, P297

[3] SEQUENCE AND ORGANIZATION OF THE HUMAN MITOCHONDRIAL GENOME [J].

ANDERSON, S ;

BANKIER, AT ;

BARRELL, BG ;

DEBRUIJN, MHL ;

COULSON, AR ;

DROUIN, J ;

EPERON, IC ;

NIERLICH, DP ;

ROE, BA ;

SANGER, F ;

SCHREIER, PH ;

SMITH, AJH ;

STADEN, R ;

YOUNG, IG .

NATURE, 1981, 290 (5806) :457-465

[4] DO NONSPERMATOZOAL CELLS MAINLY STEM FROM SPERMIOGENESIS - STUDY OF 106 FERTILE AND 102 SUBFERTILE MEN [J].

AUROUX, M ;

COLLIN, C ;

COUVILLERS, ML .

ARCHIVES OF ANDROLOGY, 1985, 14 (01) :73-80

[5] Fatal neonatal liver failure and depletion of mitochondrial DNA in three children of one family [J].

Bakker, HD ;

VandenBogert, C ;

Scholte, HR ;

Zwart, R ;

Wijburg, FA ;

Spelbrink, JN .

JOURNAL OF INHERITED METABOLIC DISEASE, 1996, 19 (02) :112-114

[6] Depletion of mitochondrial deoxyribonucleic acid in a family with fatal neonatal liver disease [J].

Bakker, HD ;

Scholte, HR ;

Dingemans, KP ;

Spelbrink, JN ;

Wijburg, FA ;

VandenBogert, C .

JOURNAL OF PEDIATRICS, 1996, 128 (05) :683-687

[7] PREPARATION OF SPERMATOGENIC CELL-POPULATIONS AT SPECIFIC STAGES OF DIFFERENTIATION IN THE HUMAN [J].

BLANCHARD, Y ;

LAVAULT, MT ;

QUERNEE, D ;

LELANNOU, D ;

LOBEL, B ;

LESCOAT, D .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 1991, 30 (03) :275-282

[8] Clinical heterogeneity associated with mitochondrial DNA depletion in muscle [J].

Campos, Y ;

Martín, MA ;

García-Silva, T ;

del Hoyo, P ;

Rubio, JC ;

Castro-Gago, M ;

García-Peñas, J ;

Casas, J ;

Cabello, A ;

Ricoy, JR ;

Arenas, J .

NEUROMUSCULAR DISORDERS, 1998, 8 (08) :568-573

[9]

Cummins JM, 1998, INT J ANDROL, V21, P47

[10] MITOCHONDRIAL DISEASE AND REDUCED SPERM MOTILITY [J].

FOLGERO, T ;

BERTHEUSSEN, K ;

LINDAL, S ;

TORBERGSEN, T ;

OIAN, P .

HUMAN REPRODUCTION, 1993, 8 (11) :1863-1868

← 1 2 3 4 5 →