Studies on the recycling of the transferrin receptor in Trypanosoma brucei using an inducible gene expression system

被引：34

作者：

Kabiri, M ^{[1
]}

Steverding, D ^{[1
]}

机构：

[1] Univ Heidelberg, Inst Hyg, Abt Parasitol, D-69120 Heidelberg, Germany

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2000年 / 267卷 / 11期

关键词：

Trypanosoma brucei; transferrin receptor; inducible gene expression; receptor recycling;

D O I：

10.1046/j.1432-1327.2000.01361.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uptake of host transferrin in bloodstream forms of Trypanosoma brucei is mediated by a heterodimeric, glycosylphosphatidylinositol-anchored receptor. After endocytosis, transferrin is delivered to lysosomes where it is proteolytically degraded. Whether the heterodimeric transferrin receptor is returned to mediate several cycles in ligand uptake is undefined. By using an inducible gene expression system we provide evidence for recycling of the transferrin receptor in bloodstream forms of T. brucei. The metabolic half-life of the transferrin receptor in bloodstream-form trypanosomes is determined to be 7 h which is comparable to the half-lives of recycling receptors in mammalian cells. The cycling time of the trypanosomal transferrin receptor is calculated to be 11 min. By means of the half-life and the cycling time, we calculated that each receptor is recycled 60 times before being degraded on average.

引用

页码：3309 / 3314

页数：6

共 33 条

[1] Transcription of 'inactive' expression sites in African trypanosomes leads to expression of multiple transferrin receptor RNAs in bloodstream forms [J].

Ansorge, I ;

Steverding, D ;

Melville, S ;

Hartmann, C ;

Clayton, C .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1999, 101 (1-2) :81-94

[2] CULTIVATION IN A SEMI-DEFINED MEDIUM OF ANIMAL INFECTIVE FORMS OF TRYPANOSOMA-BRUCEI, TRYPANOSOMA-EQUIPERDUM, TRYPANOSOMA-EVANSI, TRYPANOSOMA-RHODESIENSE AND T-GAMBIENSE [J].

BALTZ, T ;

BALTZ, D ;

GIROUD, C ;

CROCKETT, J .

EMBO JOURNAL, 1985, 4 (05) :1273-1277

[3] MONENSIN INTERRUPTS THE RECYCLING OF LOW-DENSITY LIPOPROTEIN RECEPTORS IN HUMAN-FIBROBLASTS [J].

BASU, SK ;

GOLDSTEIN, JL ;

ANDERSON, RGW ;

BROWN, MS .

CELL, 1981, 24 (02) :493-502

[4] Vectors for inducible expression of toxic gene products in bloodstream and procyclic Trypanosoma brucei [J].

Biebinger, S ;

Wirtz, LE ;

Lorenz, P ;

Clayton, C .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 85 (01) :99-112

[5] FATE OF RECEPTOR AND LIGAND DURING ENDOCYTOSIS OF ASIALOGLYCOPROTEINS BY ISOLATED HEPATOCYTES [J].

BRIDGES, K ;

HARFORD, J ;

ASHWELL, G ;

KLAUSNER, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :350-354

[6] EXPRESSION OF A GLYCOSYLPHOSPHATIDYLINOSITOL-ANCHORED TRYPANOSOMA-BRUCEI TRANSFERRIN-BINDING PROTEIN COMPLEX IN INSECT CELLS [J].

CHAUDHRI, M ;

STEVERDING, D ;

KITTELBERGER, D ;

TJIA, S ;

OVERATH, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) :6443-6447

[7]

CIECHANOVER A, 1983, J BIOL CHEM, V258, P9681

[8] COORDINATE TRANSCRIPTION OF VARIANT SURFACE GLYCOPROTEIN GENES AND AN EXPRESSION SITE ASSOCIATED GENE FAMILY IN TRYPANOSOMA BRUCEI [J].

CULLY, DF ;

IP, HS ;

CROSS, GAM .

CELL, 1985, 42 (01) :173-182

[9] ACID-DEPENDENT LIGAND DISSOCIATION AND RECYCLING OF LDL RECEPTOR MEDIATED BY GROWTH-FACTOR HOMOLOGY REGION [J].

DAVIS, CG ;

GOLDSTEIN, JL ;

SUDHOF, TC ;

ANDERSON, RGW ;

RUSSELL, DW ;

BROWN, MS .

NATURE, 1987, 326 (6115) :760-765

[10] RNA TURNOVER IN TRYPANOSOMA-BRUCEI [J].

EHLERS, B ;

CZICHOS, J ;

OVERATH, P .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (03) :1242-1249

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