Functional cross-talk among Rad51, Rad54, and replication protein A in heteroduplex DNA joint formation

被引：53

作者：

Van Komen, S ^{[1
]}

Petukhova, G ^{[1
]}

Sigurdsson, S ^{[1
]}

Sung, P ^{[1
]}

机构：

[1] Univ Texas, Hlth Sci Ctr, Inst Biotechnol, Dept Mol Med, San Antonio, TX 78245 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2002年 / 277卷 / 46期

关键词：

D O I：

10.1074/jbc.M205864200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Saccharomyces cerevisiae Rad51, Rad54, and replication protein A (RPA) proteins work in concert to make heteroduplex DNA joints during homologous recombination. With plasmid length DNA substrates, maximal DNA joint formation is observed with amounts of Rad51. substantially below what is needed to saturate the initiating single-stranded DNA template, and, relative to Rad51, Rad54 is needed in only catalytic quantities. RPA is still indispensable for optimal reaction efficiency, but its role in this instance is to sequester free single-stranded DNA, which otherwise inhibits Rad51 and Rad54 functions. We also demonstrate that Rad54 helps overcome various reaction constraints in DNA joint formation. These results thus shed light on the function of Rad54 in the Rad51-mediated homologous DNA pairing reaction and also reveal a novel role of RPA in the presynaptic stage of this reaction.

引用

页码：43578 / 43587

页数：10

共 29 条

[1] RMPs: Recombination/replication mediator proteins [J].

Beernink, HTH ;

Morrical, SW .

TRENDS IN BIOCHEMICAL SCIENCES, 1999, 24 (10) :385-389

[2]

Bianco P. R., 1998, FRONT BIOSCI, V3, pD570, DOI DOI 10.2741/A304

[3] Recombinational repair in yeast: Functional interactions between Rad51 and Rad54 proteins [J].

Clever, B ;

Interthal, H ;

SchmuckliMaurer, J ;

King, J ;

Sigrist, M ;

Heyer, WD .

EMBO JOURNAL, 1997, 16 (09) :2535-2544

[4] Recombination at double-strand breaks and DNA ends: Conserved mechanisms from phage to humans [J].

Cromie, GA ;

Connelly, JC ;

Leach, DRF .

MOLECULAR CELL, 2001, 8 (06) :1163-1174

[5] DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis [J].

Dasika, GK ;

Lin, SCJ ;

Zhao, S ;

Sung, P ;

Tomkinson, A ;

Lee, EYHP .

ONCOGENE, 1999, 18 (55) :7883-7899

[6] Direct association between the yeast Rad51 and Rad54 recombination proteins [J].

Jiang, H ;

Xie, YQ ;

Houston, P ;

Stemkehale, K ;

Mortensen, UH ;

Rothstein, R ;

Kodadek, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (52) :33181-33186

[7] Molecular mechanisms of DNA double-strand break repair [J].

Kanaar, R ;

Hoeijmakers, JHJ ;

van Gent, DC .

TRENDS IN CELL BIOLOGY, 1998, 8 (12) :483-489

[8] Rad54 protein is targeted to pairing loci by the Rad51 nucleoprotein filament [J].

Mazin, AV ;

Bornarth, CJ ;

Solinger, JA ;

Heyer, WD ;

Kowalczykowski, SC .

MOLECULAR CELL, 2000, 6 (03) :583-592

[9] The function of the secondary DNA-binding site of RecA protein during DNA strand exchange [J].

Mazin, AV ;

Kowalczykowski, SC .

EMBO JOURNAL, 1998, 17 (04) :1161-1168

[10] BRCA2 is required for homology-directed repair of chromosomal breaks [J].

Moynahan, ME ;

Pierce, AJ ;

Jasin, M .

MOLECULAR CELL, 2001, 7 (02) :263-272

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