Trefoil Factor-1 (TFF1) Enhances Oncogenicity of Mammary Carcinoma Cells

被引：56

作者：

Amiry, Naeem ^{[1
]}

Kong, Xiangjun ^{[3
,4
]}

Muniraj, Nethaji ^{[1
]}

Kannan, Nagarajan ^{[1
]}

Grandison, Prudence M. ^{[1
]}

Lin, Juan ^{[3
,4
]}

Yang, Yulu ^{[3
,4
]}

Vouyovitch, Cecile M. ^{[5
]}

Borges, Sahra ^{[5
]}

Perry, Jo K. ^{[1
]}

Mertani, Hichem C. ^{[5
]}

Zhu, Tao ^{[3
,4
]}

Liu, Dongxu ^{[1
]}

Lobie, Peter E. ^{[1
,2
]}

机构：

[1] Univ Auckland, Liggins Inst, Auckland 1023, New Zealand

[2] Univ Auckland, Fac Med & Hlth Sci, Dept Mol Med & Pathol, Auckland 1023, New Zealand

[3] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China

[4] Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China

[5] Univ Lyon 1, CNRS, UMR 5123, F-69622 Villeurbanne, France

来源：

ENDOCRINOLOGY | 2009年 / 150卷 / 10期

基金：

中国国家自然科学基金;

关键词：

BREAST-CANCER-CELLS; HUMAN GROWTH-HORMONE; EPITHELIAL-MESENCHYMAL TRANSITION; PS2; PROTEIN; MESSENGER-RNA; E-CADHERIN; SPASMOLYTIC POLYPEPTIDE; MOLECULAR-MECHANISMS; SIGNALING PATHWAYS; MARKER GENES;

D O I：

10.1210/en.2009-0066

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The functional role of autocrine trefoil factor-1 (TFF1) in mammary carcinoma has not been previously elucidated. Herein, we demonstrate that forced expression of TFF1 in mammary carcinoma cells resulted in increased total cell number as a consequence of increased cell proliferation and survival. Forced expression of TFF1 enhanced anchorage-independent growth and promoted scattered cell morphology with increased cell migration and invasion. Moreover, forced expression of TFF1 increased tumor size in xenograft models. Conversely, RNA interference-mediated depletion of TFF1 in mammary carcinoma cells significantly reduced anchorage-independent growth and migration. Furthermore, neutralization of secreted TFF1 protein by polyclonal antibody decreased mammary carcinoma cell viability in vitro and resulted in regression of mammary carcinoma xenografts. We have therefore demonstrated that TFF1 possesses oncogenic functions in mammary carcinoma cells. Functional antagonism of TFF1 can therefore be considered as a novel therapeutic strategy for mammary carcinoma. (Endocrinology 150: 4473-4483, 2009)

引用

页码：4473 / 4483

页数：11

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