Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis

被引：38

作者：

ProdHom, G

Guilhot, C

Gutierrez, MC

Varnerot, A

Gicquel, B

Vincent, V

机构：

[1] INST PASTEUR,CTR NACL REFERENCE MYCOBACTERIES,PARIS,FRANCE

[2] INST PASTEUR,UNITE GENET MYCOBACTERIENNE,PARIS,FRANCE

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 12期

关键词：

D O I：

10.1128/JCM.35.12.3331-3334.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.

引用

页码：3331 / 3334

页数：4

共 17 条

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