Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

被引：595

作者：

Zong, Yuan ^{[1
,2
]}

Wang, Yanpeng ^{[1
,2
]}

Li, Chao ^{[1
,2
]}

Zhang, Rui ^{[1
]}

Chen, Kunling ^{[1
]}

Ran, Yidong ^{[3
]}

Qiu, Jin-Long ^{[4
]}

Wang, Daowen ^{[1
]}

Gao, Caixia ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

[3] Genovo Biotechnol Co Ltd, Tianjin, Peoples R China

[4] Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing, Peoples R China

来源：

NATURE BIOTECHNOLOGY | 2017年 / 35卷 / 05期

基金：

中国国家自然科学基金;

关键词：

TARGETED MUTAGENESIS; EFFICIENT; CAS9; DNA;

D O I：

10.1038/nbt.3811

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.

引用

页码：438 / +

页数：4

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