THz time domain spectroscopy of biomolecular conformational modes

被引:273
作者
Markelz, A [1 ]
Whitmire, S
Hillebrecht, J
Birge, R
机构
[1] SUNY Buffalo, Dept Phys, Buffalo, NY 14260 USA
[2] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA
关键词
D O I
10.1088/0031-9155/47/21/318
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We discuss the use of terahertz time domain spectroscopy for studies of conformational flexibility and conformational change in biomolecules. Protein structural dynamics are vital to biological function with protein flexibility affecting enzymatic reaction rates and sensory transduction cycling times. Conformational mode dynamics occur on the picosecond timescale and with the collective vibrational modes associated with these large scale structural motions in the 1-100 cm(-1) range. We have performed THz time domain spectroscopy (TTDS) of several biomolecular systems to explore the sensitivity of TTDS to distinguish different molecular species, different Mutations within a single species and different conformations of a given biomolecule. We compare the measured absorbances to normal mode calculations and find that the TTDS absorbance reflects the density of normal modes determined by molecular mechanics calculations, and is sensitive to both conformation and mutation. These early studies demonstrate some of the advantages and limitations of using TTDS for the study of biomolecules.
引用
收藏
页码:3797 / 3805
页数:9
相关论文
共 27 条
[1]   Biomolecular electronics: Protein-based associative processors and volumetric memories [J].
Birge, RR ;
Gillespie, NB ;
Izaguirre, EW ;
Kusnetzow, A ;
Lawrence, AF ;
Singh, D ;
Song, QW ;
Schmidt, E ;
Stuart, JA ;
Seetharaman, S ;
Wise, KJ .
JOURNAL OF PHYSICAL CHEMISTRY B, 1999, 103 (49) :10746-10766
[2]   Label-free probing of the binding state of DNA by time-domain terahertz sensing [J].
Brucherseifer, M ;
Nagel, M ;
Bolivar, PH ;
Kurz, H ;
Bosserhoff, A ;
Büttner, R .
APPLIED PHYSICS LETTERS, 2000, 77 (24) :4049-4051
[3]   Electro-optic transceivers for terahertz-wave applications [J].
Chen, Q ;
Tani, M ;
Jiang, ZP ;
Zhang, XC .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 2001, 18 (06) :823-831
[4]   INTERHELICAL EFFECTS ON THE LOW-FREQUENCY MODES AND PHASE-TRANSITIONS OF LI-DNA AND NA-DNA [J].
DEMARCO, C ;
LINDSAY, SM ;
POKORNY, M ;
POWELL, J ;
RUPPRECHT, A .
BIOPOLYMERS, 1985, 24 (11) :2035-2040
[5]   THERMAL MOTIONS AND FUNCTION OF BACTERIORHODOPSIN IN PURPLE MEMBRANES - EFFECTS OF TEMPERATURE AND HYDRATION STUDIED BY NEUTRON-SCATTERING [J].
FERRAND, M ;
DIANOUX, AJ ;
PETRY, W ;
ZACCAI, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9668-9672
[6]  
GRISCHKOWSKI D, 1991, OSA P PICOSECOND ELE
[7]   Terahertz imaging via electrooptic effect [J].
Jiang, ZP ;
Zhang, XC .
IEEE TRANSACTIONS ON MICROWAVE THEORY AND TECHNIQUES, 1999, 47 (12) :2644-2650
[8]  
KATZENELLENBOGE.N, 1993, ULTRA WIDEBAND SHORT
[9]   THE ORIGIN OF THE A TO B TRANSITION IN DNA FIBERS AND FILMS [J].
LINDSAY, SM ;
LEE, SA ;
POWELL, JW ;
WEIDLICH, T ;
DEMARCO, C ;
LEWEN, GD ;
TAO, NJ ;
RUPPRECHT, A .
BIOPOLYMERS, 1988, 27 (06) :1015-1043
[10]   Pulsed terahertz spectroscopy of DNA, bovine serum albumin and collagen between 0.1 and 2.0 THz [J].
Markelz, AG ;
Roitberg, A ;
Heilweil, EJ .
CHEMICAL PHYSICS LETTERS, 2000, 320 (1-2) :42-48