The C-terminal proline-rich tail of human immunodeficiency virus type 2 Vpx is necessary for nuclear localization of the viral preintegration complex in nondividing cells
被引:65
作者:
Pancio, HA
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA
Pancio, HA
Vander Heyden, N
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA
Vander Heyden, N
Ratner, L
论文数: 0引用数: 0
h-index: 0
机构:Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA
Ratner, L
机构:
[1] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA
[2] Washington Univ, Sch Med, Dept Pathol, St Louis, MO 63110 USA
[3] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA
Human immunodeficiency virus type 2 (HIV-2), like other lentiviruses, is capable of infecting nondividing T cells and macrophages. The present work shows that in HIV-2-infected cells, Vpx is necessary for efficient nuclear import of the preintegration complex. In agreement with this finding, the subcellular Localization of a GFP-Vpx fusion protein was found to be predominantly nuclear, However, deletion of the proline-rich C-terminal 11 residues of Vpx resulted in a shift of the fusion protein to the cytoplasm, Furthermore, the same deletion in the context of the provirus resulted in a decrease in nuclear import of the preintegration complex and attenuated replication in macrophages.
机构:
HARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USAHARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USA
Bedford, MT
Chan, DC
论文数: 0引用数: 0
h-index: 0
机构:
HARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USAHARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USA
Chan, DC
Leder, P
论文数: 0引用数: 0
h-index: 0
机构:
HARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USAHARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USA
机构:
HARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USAHARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USA
Bedford, MT
Chan, DC
论文数: 0引用数: 0
h-index: 0
机构:
HARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USAHARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USA
Chan, DC
Leder, P
论文数: 0引用数: 0
h-index: 0
机构:
HARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USAHARVARD UNIV, SCH MED, HOWARD HUGHES MED INST, DEPT GENET, BOSTON, MA 02115 USA