Stability and polymerase recognition of pyridine nucleobase analogues: Role of minor-groove H-bond acceptors

被引：53

作者：

Kim, Yoonkyung ^{[1
]}

Leconte, Aaron M. ^{[1
]}

Hari, Yoshiyuki ^{[1
]}

Romesberg, Floyd E. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 46期

关键词：

DNA replication; hydrophobic effects; nucleobases; polymerases; pyridines;

D O I：

10.1002/anie.200602579

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the groove: In an effort to develop unnatural base pairs, pyridyl nucleoside analogues were synthesized and characterized (see structures). An α-glycosidic nitrogen atom provides an H-bond acceptor that does not significantly facilitate pairing with natural nucleobases. However, it forms minor-groove H-bonds with water molecules and DNA polymerases that optimize the stability and replication, respectively, of the unnatural base pair. (Figure Presented). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7809 / 7812

页数：4

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