Rapid genome sequencing of RNA viruses

被引：33

作者：

Mizutani, Tetsuya

Endoh, Daiji

Okamoto, Michiko

Shirato, Kazuya

Shimizu, Hiroyuki

Arita, Minetaro

Fukushi, Shuetsu

Saijo, Masayuki

Sakai, Kouji

Lim, Chang Kweng

Ito, Mikako

Nerome, Reiko

Takasaki, Tomohiko

Ishii, Koji

Suzuki, Tetsuro

Kurane, Ichiro

Morikawa, Shigeru

Nishimura, Hidekazu

机构：

[1] Natl Inst Infect Dis, Dept Virol 1, Musashimurayama City, Tokyo 2080011, Japan

[2] Rakuno Gakuen Univ, Ebetsu, Hokkaido, Japan

[3] Sendai Med Ctr, Sendai, Miyagi, Japan

来源：

EMERGING INFECTIOUS DISEASES | 2007年 / 13卷 / 02期

关键词：

D O I：

10.3201/eid1302.061032

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.

引用

页码：322 / 324

页数：3

共 4 条

[1] Species-independent detection of RNA virus by representational difference analysis using non-ribosomal hexanucleotides for reverse transcription [J].