RECOMBINANT PROTEIN PRODUCTION BY TRANSIENT GENE TRANSFER INTO MAMMALIAN CELLS

被引：63

作者：

Geisse, Sabine ^{[1
]}

Fux, Cornelia ^{[2
]}

机构：

[1] Novartis Inst BioMed Res, Dept NBC PPA, Basel, Switzerland

[2] Novartis Pharma AG, Dept NBx PSD Scale Up, Basel, Switzerland

来源：

GUIDE TO PROTEIN PURIFICATION, SECOND EDITION | 2009年 / 463卷

关键词：

EPSTEIN-BARR-VIRUS; SODIUM-BUTYRATE; HEK-293; CELLS; EXPRESSION; CHO; DNA; POLYETHYLENIMINE; SUSPENSION; DELIVERY; PLASMID;

D O I：

10.1016/S0076-6879(09)63015-9

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The timely availability of recombinant proteins in sufficient quantity and of validated quality is of utmost importance in driving drug discovery and the development of low molecular weight compounds, as well as for biotherapeutics. Transient gene expression (TGE) in mammalian cells has emerged as a promising technology for protein generation over the past decade as TGE meets all the prerequisites with respect to quantity and quality of the product as well as cost-effectiveness and speed of the process. Optimized protocols have been developed for both HEK293 and CHO cell lines which allow protein production at any desired scale up to >100 l and in milligram to gram quantities. Along with an overview on current scientific and technological knowledge, detailed protocols for expression of recombinant proteins on small, medium, and large scale are discussed in the following chapter.

引用

页码：223 / 238

页数：16

共 58 条

[1]

Valproic acid: A viable alternative to sodium butyrate for enhancing protein expression in mammalian cell cultures [J].

Backliwal, Gaurav ;

Hildinger, Markus ;

Kuettel, Ivan ;

Delegrange, Fanny ;

Hacker, David L. ;

Wurm, Florian M. .

BIOTECHNOLOGY AND BIOENGINEERING, 2008, 101 (01) :182-189

[2]

High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI [J].

Backliwal, Gaurav ;

Hildinger, Markus ;

Hasija, Vivek ;

Wurm, Florian M. .

BIOTECHNOLOGY AND BIOENGINEERING, 2008, 99 (03) :721-727

[3]

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions [J].

Backliwal, Gaurav ;

Hildinger, Markus ;

Chenuet, Sebastien ;

Wulhfard, Sarah ;

De Jesus, Maria ;

Wurm, Florian M. .

NUCLEIC ACIDS RESEARCH, 2008, 36 (15)

[4]

Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives [J].

Baldi, Lucia ;

Hacker, David L. ;

Adam, Myriam ;

Wurm, Florian M. .

BIOTECHNOLOGY LETTERS, 2007, 29 (05) :677-684

[5]

Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection [J].

Batard, P ;

Jordan, M ;

Wurm, F .

GENE, 2001, 270 (1-2) :61-68

[6]

Disassembly of polyethylenimine-DNA particles in vitro: Implications for polyethylenimine-mediated DNA delivery [J].

Bertschinger, Martin ;

Backliwal, Gaurav ;

Schertenleib, Arnaud ;

Jordan, Martin ;

Hacker, David L. ;

Wurm, Florian M. .

JOURNAL OF CONTROLLED RELEASE, 2006, 116 (01) :96-104

[7]

A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[8]

Calcium Phosphate Transfection Generates Mammalian Recombinant Cell Lines With Higher Specific Productivity Than Polyfection [J].

Chenuet, Sebastien ;

Martinet, Danielle ;

Besuchet-Schirultz, Nathalie ;

Wicht, Marianne ;

Jaccard, Nicolas ;

Bon, Anne-Charlotte ;

Derouazi, Madiha ;

Hacker, David L. ;

Beckmann, Jacques S. ;

Wurm, Florian M. .

BIOTECHNOLOGY AND BIOENGINEERING, 2008, 101 (05) :937-945

[9]

Cho BC, 2000, J KOREAN PHYS SOC, V37, P23

[10]

Establishment of a human somatic hybrid cell line for recombinant protein production [J].

Cho, MS ;

Yee, H ;

Chan, S .

JOURNAL OF BIOMEDICAL SCIENCE, 2002, 9 (06) :631-638

← 1 2 3 4 5 6 →