RNA-directed DNA methylation in Arabidopsis

被引：192

作者：

Aufsatz, W ^{[1
]}

Mette, MF ^{[1
]}

van der Winden, J ^{[1
]}

Matzke, AJM ^{[1
]}

Matzke, M ^{[1
]}

机构：

[1] Austrian Acad Sci, Inst Mol Biol, A-5020 Salzburg, Austria

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2002年 / 99卷

关键词：

D O I：

10.1073/pnas.162371499

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In plants, double-stranded RNA that is processed to short RNAs approximate to21-24 nt in length can trigger two types of epigenetic gene silencing. Posttranscriptional gene silencing, which is related to RNA interference in animals and quelling in fungi, involves targeted elimination of homologous mRNA in the cytoplasm. RNA-directed DNA methylation involves de novo methylation of almost all cytosine residues within a region of RNA-DNA sequence identity. RNA-directed DNA methylation is presumed to be responsible for the methylation observed in protein coding regions of post-transcriptionally silenced genes. Moreover, a type of transcriptional gene silencing and de novo methylation of homologous promoters in trans can occur if a double-stranded RNA contains promoter sequences. Although RNA-directed DNA methylation has been described so far only in plants, there is increasing evidence that RNA can also target genome modifications in other organisms. To understand how RNA directs methylation to identical DNA sequences and how changes in chromatin configuration contribute to initiating or maintaining DNA methylation induced by RNA, a promoter double-stranded RNA-mediated transcriptional gene silencing system has been established in Arabidopsis. A genetic analysis of this system is helping to unravel the relationships among RNA signals, DNA methylation, and chromatin structure.

引用

页码：16499 / 16506

页数：8

共 61 条

[1] Chromodomains are protein-RNA interaction modules [J].

Akhtar, A ;

Zink, D ;

Becker, PB .

NATURE, 2000, 407 (6802) :405-409

[2] Disruption of the plant gene MOM releases transcriptional silencing of methylated genes [J].

Amedeo, P ;

Habu, Y ;

Afsar, K ;

Scheid, OM ;

Paszkowski, J .

NATURE, 2000, 405 (6783) :203-206

[3] Arabidopsis cmt3 chromomethylase mutations block non-CG methylation and silencing of an endogenous gene [J].

Bartee, L ;

Malagnac, F ;

Bender, J .

GENES & DEVELOPMENT, 2001, 15 (14) :1753-1758

[4] Two Arabidopsis methylation-deficiency mutations confer only partial effects on a methylated endogenous gene family [J].

Bartee, L ;

Bender, J .

NUCLEIC ACIDS RESEARCH, 2001, 29 (10) :2127-2134

[5]

Bernstein E, 2001, RNA, V7, P1509

[6] The DNA methyltransferases of mammals [J].

Bestor, TH .

HUMAN MOLECULAR GENETICS, 2000, 9 (16) :2395-2402

[7] Conserved plant genes with similarity to mammalian de novo DNA methyltransferases [J].

Cao, XF ;

Springer, NM ;

Muszynski, MG ;

Phillips, RL ;

Kaeppler, S ;

Jacobsen, SE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (09) :4979-4984

[8] Paramutation in maize [J].

Chandler, VL ;

Eggleston, WB ;

Dorweiler, JE .

PLANT MOLECULAR BIOLOGY, 2000, 43 (2-3) :121-145

[9] FUNCTIONAL-ANALYSIS OF REGULATORY ELEMENTS IN A PLANT EMBRYO-SPECIFIC GENE [J].

CHEN, ZL ;

SCHULER, MA ;

BEACHY, RN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8560-8564

[10] Characteristics of post-transcriptional gene silencing [J].

Chicas, A ;

Macino, G .

EMBO REPORTS, 2001, 2 (11) :992-996

← 1 2 3 4 5 6 7 →