NoRC-dependent nucleosome positioning silences rRNA genes

被引：88

作者：

Li, Junwei

Laengst, Gernot

Grummt, Ingrid

机构：

[1] German Canc Res Ctr, Div Mol Biol Cell 2, D-69120 Heidelberg, Germany

[2] Inst Biochem Genet & Mikrobiol, Regensburg, Germany

来源：

EMBO JOURNAL | 2006年 / 25卷 / 24期

关键词：

differentiation; NoRC; nucleosome positioning; RNA polymerase I; TIP5;

D O I：

10.1038/sj.emboj.7601454

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have established that the Snf2h-containing chromatin remodeling complex NoRC mediates epigenetic silencing of a subset of rRNA genes ( rDNA) by recruiting enzymatic activities that modify histones and methylate DNA. Here we have analyzed nucleosome positions at the murine rDNA promoter and show that active and silent rDNA copies are characterized not only by specific epigenetic marks but also by differently positioned nucleosomes. At active genes the promoter-bound nucleosome covers nucleotides from -157 to -2, whereas at silent genes the nucleosome is positioned 25 nucleotides further downstream. We provide evidence that NoRC is the molecular machine that shifts the promoter-bound nucleosome downstream of the transcription start site into a translational position that is unfavorable for transcription complex formation.

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收藏

页码：5735 / 5741

页数：7

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