Spatiotemporal change of rat collagenase (MMP-13) mRNA expression in the development of the rat femoral neck

被引:20
作者
Hayami, T
Endo, N
Tokunaga, K
Yamagiwa, H
Hatano, H
Uchida, M
Takahashi, HE
机构
[1] Niigata Univ, Sch Med, Dept Orthoped Surg, Niigata 9518510, Japan
[2] Kurcha Chem Ind Co, Biomed Res Labs, Tokyo, Japan
[3] Niigata Bone Sci Inst, Niigata, Japan
关键词
femoral neck; matrix metalloprotease (MMP)-13; bone modeling; in situ hybridization; histomorphometry;
D O I
10.1007/s007740070019
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The interepiphyseal region between the greater trochanter and the capital femoral epiphysis and the medioproximal portion of the femoral neck exhibit extensive morphological changes during the first 4 weeks after birth in rats. Previous reports show that matrix metalloproteinase-13 (MMP-13, rat collagenase) mRNA is expressed in bone and cartilage during embryonal development and fracture healing. We examined MMP-13 mRNA expression and compared it with the distribution of osteopontin and osteocalcine mRNA in the femoral neck. Moreover, we examined histomorphometric analysis in the femoral neck where the morphology changes rapidly. Histomorphometric analysis of the 4-week-old rat femoral neck showed a high rate of bone formation and resorption in the region where shape changed rapidly. Osteopontin mRNA was expressed diffusely along the endosteum. In contrast, MMP-13 mRNA expression was restricted to the medial endosteal portion near the cartilage-bone interlace of the femoral neck in 15- and 28-day-old rats and in the deepest endosteal interepiphyseal region of 15-day-old rats. MMP-13 mRNA-expressing osteoblastic cells were also expressing osteopontin but not osteocalcin mRNA. MMP-13 mRNA-expressing cells differ from tartrate-resistant acid phospatase (TRAP) -positive cells, and MMP-13 mRNA-positive cells are located. adjacent to TRAP-positive cells. The results of the site- and cell-specific expression of MMF-13, taken together with its enzymatic property, suggest that MMP-13 plays an important role in morphological changes in the rat femur, at least during the third and fourth week after birth, and that MMP-13 itself is involved in the interaction between osteoblastic and TRAP-positive cells.
引用
收藏
页码:185 / 193
页数:9
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