Uses of plasmid technologies .1. The role of oestrogen in follicular development

被引：12

作者：

Hurst, BS

Leslie, KK

机构：

[1] UNIV COLORADO,HLTH SCI CTR,DEPT OBSTET & GYNECOL,DIV MATERNAL FETAL MED,DENVER,CO 80262

[2] UNIV COLORADO,HLTH SCI CTR,REPROD MOL LAB,DENVER,CO 80262

来源：

MOLECULAR HUMAN REPRODUCTION | 1997年 / 3卷 / 08期

关键词：

granulosa cells; oestrogen receptor; oestrogen responsive element; ovary; reverse transcriptase PCR;

D O I：

10.1093/molehr/3.8.643

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Most of our knowledge of ovarian physiology is based upon studies that have demonstrated functional oestrogen receptors in the ovaries of lower animal species. The presence of oestrogen receptors in primate granulosa cells has been questioned by some investigators. However, we have found oestrogen receptor messenger RNA in human granulosa cells by reverse transcriptase-PCR assay. Furthermore, using immortalized granulosa cell lines transfected with a plasmid containing an oestrogen response element, a functional oestrogen receptor was confirmed. These experiments strongly support the hypothesis that human granulosa cells express biologically active oestrogen receptor.

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页码：643 / 645

页数：3

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