Fibroblast growth factor receptor-1 is expressed by endothelial progenitor cells

被引:66
作者
Burger, PE
Coetzee, S
McKeehan, WL
Kan, M
Cook, P
Fan, Y
Suda, T
Hebbel, RP
Novitzky, N
Muller, WA
Wilson, EL
机构
[1] Univ Cape Town, Dept Immunol, ZA-7925 Cape Town, South Africa
[2] Univ Cape Town, Dept Hematol, ZA-7925 Cape Town, South Africa
[3] NYU, Med Ctr, Dept Cell Biol, New York, NY 10016 USA
[4] NYU, Med Ctr, Dept Med, New York, NY 10016 USA
[5] NYU, Med Ctr, Kaplan Canc Ctr, New York, NY 10016 USA
[6] Texas A&M Univ, Syst Hlth Sci Ctr, Inst Biosci & Technol, Ctr Canc Biol & Nutr, Houston, TX USA
[7] Zeria Pharmaceut Co Ltd, Saitama, Japan
[8] Columbia Univ, Dept Transfus Med, New York, NY USA
[9] Kumamoto Univ, Dept Cell Differentiat, Kumamoto, Japan
[10] Univ Minnesota, Sch Med, Dept Med, Minneapolis, MN 55455 USA
[11] Cornell Univ, Weill Med Coll, Dept Pathol, New York, NY USA
关键词
D O I
10.1182/blood.V100.10.3527
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Recent experiments show that hematopoietic progenitor cell populations contain endothelial precursor cells. We have isolated a population of CD34(+) cells that expresses fibroblast growth factor receptor-1 (FGFR-1) and that differentiates into endothelial cells in vitro. We find that 4.5% +/- 2.1% of CD34(+) cells isolated from bone marrow, cord blood, and mobilized peripheral blood express FGFR-1 and that viable CD34(+)FGFR(+) cells are small, with little granularity, and express both primitive hematopoietic and endothelial markers on their surface. The primitive hematopoietic markers AC133, c-kit, and Thy-1 are coexpressed by 75%, 85%, and 64% of CD34(+)FGFR(+) cells, respectively. Most of the CD34(+)FGFR(+) cells also express antigens found on endothelial cells, such as CD31, vascular endothelial growth factor receptor-2, and the endothelial-specific cell surface marker, vascular endothelial cadherin (VE-cadherin), whereas 56% to 60% of the cells express Tie, Tek, and the endothelial-specific marker, P1H12. The CD34(+)FGFR(+) population is enriched in cells expressing endothelial-specific antigens compared with the CD34(+) population. Isolated CD34(+)FGFR(+) cells grow slowly in culture, are stimulated by fibroblast growth factor-2 and vascular endothelial growth factor, and give rise to cells that express von Willebrand factor and VE-cadherin and that incorporate acetylated low-density lipoprotein. These experiments show that FGFR-1 is expressed by a subpopulation of CD34(+) cells that give rise to endothelial cells in vitro, indicating that this population contains endothelial stem/progenitor cells.
引用
收藏
页码:3527 / 3535
页数:9
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