Alphaviral gene transfer in neurobiology

被引:15
作者
Ehrengruber, MU [1 ]
机构
[1] Univ Zurich, Brain Res Inst, CH-8057 Zurich, Switzerland
关键词
biosafety; expression vector; green fluorescent protein; hippocampus; Semliki Forest virus; Sindbis virus;
D O I
10.1016/S0361-9230(02)00858-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Semliki Forest virus (SFV), Sindbis virus (SIN), and Venezuelan equine encephalitis virus are simple, enveloped plus-strand RNA viruses belonging to the Alphavirus genus of the Togaviridae family. They have been developed into expression vectors that infect a wide host cell range and cause rapid and high-level transgene expression. Their easy and fast generation, classification into biosafety levels 1 and 2, and preferential transduction of neurons in cell and tissue cultures makes them an increasingly used gene transfer system. This review summarizes the alphaviral replication and expression, the replicon system, and its application in neurobiology. Alphaviral vectors can introduce multiple transgenes into host cells, and mutants with low or absent cytotoxicity and increased or decreased transgene expression levels are available. Temperature-dependent mutants permit to control the host cell specificity as well as the on- and offset of gene expression. These features, together with the transduction characteristics revealed in a direct comparison of alphaviral and other viral vectors in hippocampal slice cultures, make SFV and SIN vectors a powerful tool for neurobiological studies. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:13 / 22
页数:10
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