Response to ADP-ribose by activation of TRPM2 in the CRI-G1 insulinoma cell line

被引：78

作者：

Inamura, K ^{[1
]}

Sano, Y ^{[1
]}

Mochizuki, S ^{[1
]}

Yokoi, H ^{[1
]}

Miyake, A ^{[1
]}

Nozawa, K ^{[1
]}

Kitada, C ^{[1
]}

Matsushime, H ^{[1
]}

Furuichi, K ^{[1
]}

机构：

[1] Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Mol Med Labs, Tsukuba, Ibaraki 3058585, Japan

来源：

JOURNAL OF MEMBRANE BIOLOGY | 2003年 / 191卷 / 03期

关键词：

ADP-ribose; beta-NAD; TRPM2; CRI-G1; cell; calcium;

D O I：

10.1007/s00232-002-1057-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The response to intracellular ADP-ribose in the rat CRI-G1 insulinoma cell line was studied using a patch-clamp method. Dialysis of ADP-ribose into cells induced a response in a dose-dependent manner. The reversal potentials in various solutions showed that the ADP-ribose-gated channel was a Ca2+-permeable nonselective cation channel. In inside-out recordings, ADP-ribose and beta-NAD induced responses in the same patch. The single-channel current-voltage relationships for ADP-ribose- and beta-NAD-induced responses were almost identical, indicating that ADP-ribose and beta-NAD activated the same channel. The physiological properties of the ADP-ribose-gated channel are similar to those we reported previously for the cloned transient receptor potential channel TRPM2. Moreover, RT-PCR analysis showed that TRPM2 was abundantly expressed in CRI-G1 cells, suggesting that the ADPribose-gated channel represents the native TRPM2 channel in CRI-G1 cells. These results suggest that ADP-ribose can be an endogenous modulator of Ca2+ influx through the TRPM2 channel into CRI-G1 cells.

引用

页码：201 / 207

页数：7

共 40 条

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