Plant Peptides Govern Terminal Differentiation of Bacteria in Symbiosis

被引：398

作者：

Van de Velde, Willem ^{[1
]}

Zehirov, Grigor ^{[2
]}

Szatmari, Agnes ^{[1
,3
]}

Debreczeny, Monika ^{[4
]}

Ishihara, Hironobu ^{[2
]}

Kevei, Zoltan ^{[4
]}

Farkas, Attila ^{[4
]}

Mikulass, Kata ^{[4
]}

Nagy, Andrea ^{[4
]}

Tiricz, Hilda ^{[4
]}

Satiat-Jeunemaitre, Beatrice ^{[1
]}

Alunni, Benoit ^{[1
]}

Bourge, Mickael ^{[1
]}

Kucho, Ken-ichi ^{[2
]}

Abe, Mikiko ^{[2
]}

Kereszt, Attila ^{[4
]}

Maroti, Gergely ^{[4
]}

Uchiumi, Toshiki ^{[2
]}

Kondorosi, Eva ^{[1
,4
]}

Mergaert, Peter ^{[1
]}

机构：

[1] CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France

[2] Kagoshima Univ, Grad Sch Sci & Engn, Kagoshima 8900065, Japan

[3] Hungarian Acad Sci, Inst Plant Protect, H-1022 Budapest, Hungary

[4] Bay Zoltan Fdn Appl Res, Inst Plant Genom Human Biotechnol & Bioenergy, H-6726 Szeged, Hungary

来源：

SCIENCE | 2010年 / 327卷 / 5969期

关键词：

MEDICAGO-TRUNCATULA; LEGUME SYMBIOSIS; ANTIMICROBIAL PEPTIDES; RHIZOBIUM-MELILOTI; CELL-CYCLE; GENES; NODULES; ORGANIZATION; MODEL;

D O I：

10.1126/science.1184057

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Legume plants host nitrogen-fixing endosymbiotic Rhizobium bacteria in root nodules. In Medicago truncatula, the bacteria undergo an irreversible (terminal) differentiation mediated by hitherto unidentified plant factors. We demonstrated that these factors are nodule-specific cysteine-rich (NCR) peptides that are targeted to the bacteria and enter the bacterial membrane and cytosol. Obstruction of NCR transport in the dnf1-1 signal peptidase mutant correlated with the absence of terminal bacterial differentiation. On the contrary, ectopic expression of NCRs in legumes devoid of NCRs or challenge of cultured rhizobia with peptides provoked symptoms of terminal differentiation. Because NCRs resemble antimicrobial peptides, our findings reveal a previously unknown innovation of the host plant, which adopts effectors of the innate immune system for symbiosis to manipulate the cell fate of endosymbiotic bacteria.

引用

页码：1122 / 1126

页数：5

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