Use of integrated cell culture-PCR to evaluate the effectiveness of poliovirus inactivation by chlorine

被引:43
作者
Blackmer, F [1 ]
Reynolds, KA [1 ]
Gerba, CP [1 ]
Pepper, IL [1 ]
机构
[1] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA
关键词
D O I
10.1128/AEM.66.5.2267-2268.2000
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, integrated cell culture-PCR detected viruses for up to 8 min of exposure to the same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to five times greater than previously thought.
引用
收藏
页码:2267 / 2268
页数:2
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