Exosome-mediated delivery of RNA and DNA for gene therapy

被引：104

作者：

Munagala, Radha ^{[1
]}

Aqil, Farrukh ^{[2
,3
]}

Jeyabalan, Jeyaprakash ^{[1
]}

Kandimalla, Raghuram ^{[2
,4
]}

Wallen, Margaret ^{[1
]}

Tyagi, Neha ^{[2
,4
]}

Wilcher, Sarah ^{[5
]}

Yan, Jun ^{[2
,6
]}

Schultz, David J. ^{[7
]}

Spencer, Wendy ^{[1
]}

Gupta, Ramesh C. ^{[1
,2
,4
]}

机构：

[1] 3P Biotechnol Inc, Louisville, KY 40202 USA

[2] Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40202 USA

[3] Univ Louisville, Dept Med, Louisville, KY 40202 USA

[4] Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40202 USA

[5] Univ Louisville, Res Resources Ctr, Louisville, KY 40202 USA

[6] Univ Louisville, Dept Surg, Louisville, KY 40202 USA

[7] Univ Louisville, Dept Biol, Louisville, KY 40292 USA

来源：

CANCER LETTERS | 2021年 / 505卷

关键词：

Exosome; Cancer; Delivery; siRNA; Plasmid-DNA; TARGETING DRUG-DELIVERY; MILK-DERIVED EXOSOMES; SYSTEMIC INJECTION; CLINICAL-TRIAL; CANCER; SIRNA; BIODISTRIBUTION; ANTHOCYANIDINS; NANOPARTICLES; DOXORUBICIN;

D O I：

10.1016/j.canlet.2021.02.011

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Gene therapy promises to revolutionize biomedicine and personalized medicine by modulating or compensating the expression of abnormal genes. The biggest obstacle for clinical application is the lack of an effective, nonimmunogenic delivery system. We show that bovine colostrum exosomes and polyethyleneimine matrix (EPM) delivers short interfering RNA (siRNA) or plasmid DNA (pDNA) for effective gene therapy. KRAS, a therapeutic focus for many cancers, was targeted by EPM-delivered KRAS siRNA (siKRAS) and inhibited lung tumor growth (>70%) and reduced KRAS expression (50%-80%). Aberrant p53 is another therapeutic focus for many cancers. EPM-mediated introduction of wild-type (WT) p53 pDNA (pcDNA-p53) resulted in p53 expression in p53-null H1299 cells in culture, subcutaneous lung tumor, and tissues of p53-knockout mice. Additionally, chemosensitizing effects of paclitaxel were restored by exogenous WT p53 in lung cancer cells. Together, this novel EPM technology represents an effective `platform' for delivery of therapeutic nucleic acids to treat human disease.

引用

页码：58 / 72

页数：15

共 71 条

[1]

Milk-derived exosomes for oral delivery of paclitaxel [J].