Isolation and characterization of bile salts-sensitive mutants of Enterococcus faecalis

被引：49

作者：

Le Breton, Y ^{[1
]}

Mazé, A ^{[1
]}

Hartke, A ^{[1
]}

Lemarinier, S ^{[1
]}

Auffray, Y ^{[1
]}

Rincé, A ^{[1
]}

机构：

[1] Univ Caen, Lab Microbiol Environm, Inst Rech Biol Appl, INRA,EA 956 USC, F-14032 Caen, France

来源：

CURRENT MICROBIOLOGY | 2002年 / 45卷 / 06期

关键词：

D O I：

10.1007/s00284-002-3714-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A library of insertional mutants of Enterococcus faecalis was constructed; it allowed the isolation and the characterization of 10 mutants affected in resistance to bile salts. Insertion loci of two mutants corresponded to genes of unknown function, while the amino acid sequences deduced from the other loci were homologous to proteins related to DNA repair, oxidative response, transcriptional regulation, dGTP hydrolysis, membrane composition, or cell wall synthesis. Further characterization of one mutant revealed that the insertion within the E. faecalis sagA gene led to a decrease of the resistance towards numerous independent physicochemical stresses, to modifications of the cell wall integrity, and to perturbations of cell division with septation anomalies.

引用

页码：434 / 439

页数：6

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