Broadly reactive antibodies against a gp120 V3 loop multi-epitope polypeptide neutralize different isolates of human immunodeficiency virus type 1 (HIV-1)

被引：17

作者：

Montero, M

Menendez, A

Dominguez, MD

Navea, L

Vilarubia, OL

Quintana, D

Izquierdo, M

Jimenez, V

Reyes, O

Lobaina, L

Noa, E

Duarte, CA

机构：

[1] CTR GENET ENGN & BIOTECHNOL, VACCINE DIV, HAVANA 10600, CUBA

[2] NATL REFERENCE CTR AIDS, HAVANA, CUBA

[3] CTR GENET ENGN & BIOTECHNOL, DIV PHYS CHEM, HAVANA 10600, CUBA

来源：

VACCINE | 1997年 / 15卷 / 11期

关键词：

D O I：

10.1016/S0264-410X(97)00012-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A gene encoding for a novel multi-epitope polypeptide (TAB4) was synthesized and expressed in Escherichia coli. The protein Was composed of 15 amino acid fragments derived from the V3 loop of HIV-1 isolates MN, IIIB, RF, JY1, BRVA and LR150, joined by five-amino-acid linkers. Immunogenicity of TAB4 in rabbits was studied, and the antibody response against individual peptides investigated, TAB4 was shown to be immunogenic in Complete Freund's Adjuvant in a dose-dependent manner, and was able to elicit a humoral response against all V3 epitopes included on the protein. Sera from some of the animals were able to neutralize the replication of viral strain MN, and in one case IIIB, with moderate titers, Some ser-a also neutralized several Cuban clinical strains isolated in peripheral blood mononuclear cells; after one round of amplification in MT4 cells. (C) 1997 Elsevier Science Ltd.

引用

页码：1200 / 1208

页数：9

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