Time-dependent immunohistochemical detection of proinflammatory cytokines (IL-1β, IL-6, TNF-α) in human skin wounds

被引:101
作者
Grellner, W [1 ]
机构
[1] Univ Saarland, Inst Forens Med, D-66421 Homburg, Germany
关键词
cytokines; interleukin-1; beta; interleukin-6; tumour necrosis factor alpha; wound age; vitality; immunohistochemistry;
D O I
10.1016/S0379-0738(02)00342-0
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
The proinflammatory cytokines interleukin-1 beta (IL-1beta), interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) hold important functions in the early and late courses of inflammation, trauma and wound healing. In the present study, human skin wounds due to sharp force (n = 105) were collected during surgery and autopsy. The wound age mainly varied from several minutes to 5 h, some specimens aged up to 6 weeks. Control specimens from uninjured skin were avail able in each case. After preparation of cryostat sections, immunohistochemistry was performed according to the APAAP technique, using monoclonal and polyclonal antibodies. The results were evaluated semiquantitatively. All markers were weakly expressed in normal human skin constitutively. However, the staining pattern changed significantly in vital wounds concerning epidermal layers, subepidermal cells, vessels and sweat glands. IL-1beta and IL-6 showed enhanced expression after 15 and 20 win at the earliest (increase of epidermal reactivity). After 30-60 and 60-90 min, respectively, marked expression was observed with these markers. Similar alterations were detectable with TNF-a after 15 and 60-90 min. The reactivity of all three markers persisted over several hours, then decreased to basal levels again and sometimes reappeared after days and in granulation tissue. Leukocytes reacting with IL-1beta and IL-6 appeared after approximately 2 h. Conclusion: proinflammatory cytokines can serve as a useful tool for the estimation of vitality and wound age, in particular in the early post-traumatic interval prior to leukocyte reaction. Autolysis did not play a role in the samples investigated (postmortem interval up to 8 days). Problems could sometimes rise from constitutive expression. Therefore, it is recommended to examine control samples from the same individual and to compare the reactivity with wound specimens. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:90 / 96
页数:7
相关论文
共 43 条
[1]  
Ahmed AA, 1996, ACTA DERM-VENEREOL, V76, P348
[2]  
Amberg R., 1996, WOUND HEALING PROCES, P107
[3]  
APPLETON I, 1993, LAB INVEST, V69, P405
[4]   CYTOKINES - COORDINATORS OF IMMUNE AND INFLAMMATORY RESPONSES [J].
ARAI, K ;
LEE, F ;
MIYAJIMA, A ;
MIYATAKE, S ;
ARAI, N ;
YOKOTA, T .
ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :783-836
[5]   GROWTH-FACTORS AND WOUND-HEALING - BIOCHEMICAL-PROPERTIES OF GROWTH-FACTORS AND THEIR RECEPTORS [J].
BENNETT, NT ;
SCHULTZ, GS .
AMERICAN JOURNAL OF SURGERY, 1993, 165 (06) :728-737
[6]   GROWTH-FACTORS AND WOUND-HEALING .2. ROLE IN NORMAL AND CHRONIC WOUND-HEALING [J].
BENNETT, NT ;
SCHULTZ, GS .
AMERICAN JOURNAL OF SURGERY, 1993, 166 (01) :74-81
[7]   HISTOLOGICAL AND ENZYME-HISTOCHEMICAL PARAMETERS FOR THE AGE ESTIMATION OF HUMAN SKIN WOUNDS [J].
BETZ, P .
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 1994, 107 (02) :60-68
[8]   IMMUNOHISTOCHEMICAL PARAMETERS FOR THE AGE ESTIMATION OF HUMAN SKIN WOUNDS [J].
BETZ, P .
AMERICAN JOURNAL OF FORENSIC MEDICINE AND PATHOLOGY, 1995, 16 (03) :203-209
[9]  
BETZ P, 1993, THESIS U MUNCHEN
[10]  
BRADDING P, 1995, J IMMUNOL, V155, P297