Improved biplex quantitative real-time polymerase chain reaction with modified primers for gene expression analysis

被引：2

作者：

Afonina, Irina A. ^{[1
]}

Mills, Alan ^{[1
]}

Sanders, Silvia ^{[1
]}

Kulchenko, Alena ^{[1
]}

Dempcy, Robert ^{[1
]}

Lokhov, Sergey ^{[1
]}

Vermeulen, Nicolaas M. J. ^{[1
]}

Mahoney, Walt ^{[1
]}

机构：

[1] Nanogen Inc, Bothell, WA 98021 USA

来源：

OLIGONUCLEOTIDES | 2006年 / 16卷 / 04期

关键词：

D O I：

10.1089/oli.2006.16.395

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stabilizing modified bases incorporated in primers allows the reduction of housekeeping gene primer concentration not possible with regular primers without sacrificing amplification efficiency. Low primer concentration allows coamplification of the most abundant housekeeping genes with very rare templates without mutual inhibition. Real-time polymerase chain reaction (PCR) coamplification of 18S ribosomal RNA with several genes of interest was used in this study with MGB Eclipse (R) (Nanogen, San Diego, CA) hybridization probes. The results may be useful for high throughput gene expression studies as they simplify validation experiments.

引用

页码：395 / 403

页数：9

共 13 条

[1] Minor groove binder-conjugated DNA probes for quantitative DNA detection by hybridization-triggered fluorescence [J].