Prevalence and genotyping of human isolates of Giardia duodenalis from Albania

被引：14

作者：

Berrilli, F.

Di Cave, D.

D'Orazi, C.

Orecchia, P.

Xhelilaj, L.

Bejko, D.

Caca, P.

Bebeci, D.

Cenko, F.

Donia, D.

Divizia, M.

机构：

[1] Univ Roma Tor Vergata, Fac Med, Dept Publ Hlth & Cell Biol, I-00133 Rome, Italy

[2] Azienda Osped Univ Policlin Tor Vergata, Rome, Italy

[3] Univ Hosp Ctr Mother Theresa, Paediat Unit, Tirana, Albania

[4] Amer Red Cross, Albanian Child Survival Project, Tirana, Albania

来源：

PARASITOLOGY INTERNATIONAL | 2006年 / 55卷 / 04期

关键词：

Giardia duodenalis; genotyping; humans; Albania;

D O I：

10.1016/j.parint.2006.06.002

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Microscopical and PCR-based techniques were performed in order to investigate the prevalence of infection and the genotypes of Giardia duodenalis from 125 stool samples collected from children living in the urban and the rural areas of Tirana (Albania) and hospitalized with acute gastroenteritis. 7 out of 125 samples resulted positive for Giardia at the microscopic examination (5.6%). In 50 selected samples including the 7 samples positive for Giardia by microscopy, 3 and 15 additional positive samples were detected by immunofluorescence and PCR, respectively. Seasonality appeared as an important parameter to be evaluated in order to better understand the prevalence of infection. Sequence analysis revealed both human Assemblage A and B. This result represents the first data on G. duodenalis genotypes in Albania. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

引用

页码：295 / 297

页数：3

共 19 条

[1] Blinded application of microscopy, bacteriological culture, immunoassays and PCR to detect gastrointestinal pathogens from faecal samples of patients with community-acquired diarrhoea [J].