The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines

被引:32
作者
Castro, CP
Nagashima, AC
Pereda, MP
Goldberg, V
Chervin, A
Largen, P
Renner, U
Stalla, GK
Arzt, E
机构
[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biol, Lab Fisiol & Biol Mol, RA-1428 Buenos Aires, DF, Argentina
[2] Max Planck Inst Psychiat, Dept Endocrinol, D-80804 Munich, Germany
[3] Univ Buenos Aires, Fac Med, Inst Invest Med, RA-1427 Buenos Aires, DF, Argentina
[4] Hosp Santa Lucia, RA-1232 Buenos Aires, DF, Argentina
关键词
D O I
10.1210/en.141.5.1746
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor and interleukin-6 (IL-6), which belong to the cytokine receptor family using the common gp130 signal transducer. We studied the actions of two other members of this family, IL-11 and ciliary neurotropic factor (CNTF), on folliculostellate (FS) cells (TtT/GF cell line) and lactosomatotropic cells (GH3 cell line). The messenger RNA (mRNA) for the or-chain specific for the IL-11 receptor (1.7 kb) and CNTF receptor (2 kb) are expressed on both cell types. In addition, we detected CNTF receptor mRNA in normal rat anterior pituitary cells. IL-11 (1.25-5 nM) dose dependently stimulated the proliferation of FS cells. CNTF, at doses from 0.4-2 nM, also significantly stimulated the growth of these cells. In addition, both cytokines significantly stimulated proliferation of lactosomatotropic GH3 cells, and CNTF stimulated hormone production (GH and PRL) at 24 h by these cells. At 16-72 h, IL-11 stimulates the secretion of the angiogenic factor vascular endothelial growth factor by FS cells.In addition, both GH3 and FS cells express CNTF mRNA. These data suggest Chat IL-11 and CNTF may act as growth and regulatory factors in anterior pituitary cells.
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页码:1746 / 1753
页数:8
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