Langmuir-Blodgett films of lipase for biocatalysis

被引：14

作者：

Pastorino, L ^{[1
]}

Nicolini, C ^{[1
]}

机构：

[1] Univ Genoa, Dept Biophys M&O Sci & Technol, I-16132 Genoa, Italy

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2002年 / 22卷 / 02期

关键词：

lipase; Langmuir-Blodgett technique; biocatalysis;

D O I：

10.1016/S0928-4931(02)00197-2

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Lipase thin films were fabricated by means of the Langmuir-Blodgett (LB) technique and then structurally and functionally characterized by means of biophysical techniques toward biocatalytic applications. Protein films were studied at the air-water interface and then transferred onto a solid support by means of the horizontal lift method, namely, the Langmuir-Schaefer (LS) method. The biophysical studies were carried out mainly by means of Brewster Angle Microscopy (BAM), UV-Vis spectroscopy, quartz crystal microbalance (QCM) and circular dichroism (CD) allowed to analyze the main properties and features of such enzyme films. Finally, the functionality of lipase in the Langmuir-Blodgett films was checked with the monitoring of the enzymatic catalysis. The results indicated that the catalytic activity of the enzyme not only was preserved but also increased with respect the solution. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：419 / 422

页数：4

共 17 条

[1] HEAT-STABLE LANGMUIR-BLODGETT-FILM OF GLUTATHIONE-S-TRANSFERASE [J].

ANTOLINI, F ;

PADDEU, S ;

NICOLINI, C .

LANGMUIR, 1995, 11 (07) :2719-2725

[2]

BLECKER C, 1995, COLLOID SURFACE B, V3, P271

[3] Built-up films of barium stearate and their optical properties [J].

Blodgett, KB ;

Langmuir, I .

PHYSICAL REVIEW, 1937, 51 (11) :0964-0982

[4] EFFECT OF MONOLAYER SURFACE PRESSURE ON THE ACTIVITIES OF PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C-BETA-1, PHOSPHOLIPASE-C-GAMMA-1, AND PHOSPHOLIPASE-C-DELTA-1 [J].

BOGUSLAVSKY, V ;

REBECCHI, M ;

MORRIS, AJ ;

JHON, DY ;

RHEE, SG ;

MCLAUGHLIN, S .

BIOCHEMISTRY, 1994, 33 (10) :3032-3037

[5] NANOGRAVIMETRIC GAUGE FOR SURFACE-DENSITY MEASUREMENTS AND DEPOSITION ANALYSIS OF LANGMUIR-BLODGETT-FILMS [J].

FACCI, P ;

EROKHIN, V ;

NICOLINI, C .

THIN SOLID FILMS, 1993, 230 (02) :86-89

[6]

Garcia-Alles LF, 1998, BIOTECHNOL BIOENG, V59, P684, DOI 10.1002/(SICI)1097-0290(19980920)59:6<684::AID-BIT5>3.0.CO

[7]

2-B

[8]

GILL J, 1997, BIOCHEM EDUC, V25, P10

[9] Improving enzymes by using them in organic solvents [J].

Klibanov, AM .

NATURE, 2001, 409 (6817) :241-246

[10] Activities of urease and pepsin monolayers [J].

Langmuir, I ;

Schaefer, VJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1938, 60 :1351-1360

← 1 2 →