Porin of Shigella dysenteriae activates mouse peritoneal macrophage through Toll-like receptors 2 and 6 to induce polarized type I response

被引：32

作者：

Biswas, Amlan ^{[1
]}

Banerjee, Pallavi ^{[1
]}

Mukherjee, Gayatri ^{[1
]}

Biswas, Tapas ^{[1
]}

机构：

[1] Natl Inst Cholera & Enter Dis, Div Immunol & Vaccine Dev, Kolkata 700010, W Bengal, India

来源：

MOLECULAR IMMUNOLOGY | 2007年 / 44卷 / 05期

关键词：

porin; toll-like receptors; NF-kappa B; chemokines; macrophage polarization;

D O I：

10.1016/j.molimm.2006.04.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porin of Shigella dysenteriae type I coexpressed Toll-like receptor (TLR) 2 and TLR6 on peritoneal cavity (PerC) macrophages (M Phi) of C57BL/6 mice implicating that both the TLRs are essential as a combinatorial repertoire to recognize the protein. Besides TLRs, mRNA for MyD88 and TRAF6, and nuclear translocation of NF-kappa B were enhanced that indicate their involvement in tandem in the activity of porin. The protein selectively up-regulated CD80 on the activated M Phi together with MHC class 11 molecule and CD40, and had no effect on CD86 expression. The porin-induced profile of MIP-1 alpha, MIP-1 beta and RANTES showed strong bias for chemokines correlated with M I polarization. Intracellular expression and release of TNF-alpha and 1L-12 in presence of porin was found to be TLR2 and NF-kappa B dependent. Induction of TNF-alpha and IL-12 along with the chemokine profile suggests type I polarization of the M Phi that would influence Th1-type response. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：812 / 820

页数：9

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