Application of polypyrrole for the creation of immunosensors

被引:103
作者
Ramanaviciene, A
Ramanavicius, A
机构
[1] Inst Immunol, Lab Ecol Immunol, LT-2600 Vilnius, Lithuania
[2] Vilnius State Univ, Dept Analyt & Environm Chem, LT-2009 Vilnius, Lithuania
关键词
conducting polymers; polypyrrole; immunosensors; immobilization; antibody;
D O I
10.1080/10408340290765542
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This review focuses on the use of conducting polymer (CP) films in electrochemical affinity sensors and emphasizes innovative designs and unique applications of immunosensors. The review covers some aspects in the application of polypyrrole (Ppy) for the creation of immunosensors. Polypyrrole film fabrication methoids like solvent casting, adsorption, and electropolymerization are presented. The focus, is on electrochemically synthesized Ppy as very promising material for the formation of miniaturized electrochemical immunosensors. Polypyrrole films implemented in various capacities in amperometric, conductometric, and potentiometric immunosensor design are reviewed. The acceptance of immobilization and detection approaches used recently in affinity sensors with critical analysis applied in certain techniques is discussed. The biologically active components (BAC) used for the creation of polypyrrole-based immunosensors are described briefly. Some future trends in the development of polypyrrole-based immunosensors are predicted, as well as possible directions discussed.
引用
收藏
页码:245 / 252
页数:8
相关论文
共 50 条
[1]  
[Anonymous], [No title captured]
[2]   Secretory function of adrenal chromaffin cells cultured on polypyrrole films [J].
Aoki, T ;
Tanino, M ;
Sanui, K ;
Ogata, N ;
Kumakura, K .
BIOMATERIALS, 1996, 17 (20) :1971-1974
[3]   Direct electrochemical immunosensor for polychlorinated biphenyls [J].
Bender, S ;
Sadik, OA .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1998, 32 (06) :788-797
[4]   The new era of automated immunoassay [J].
Bock, JL .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2000, 113 (05) :628-646
[5]  
Campbell TE, 1999, ELECTROANAL, V11, P215, DOI 10.1002/(SICI)1521-4109(199904)11:4<215::AID-ELAN215>3.0.CO
[6]  
2-#
[7]   Real-time measurements of corticosteroids in conscious animals using an antibody-based electrode [J].
Cook, CJ .
NATURE BIOTECHNOLOGY, 1997, 15 (05) :467-471
[8]   Poly(pyrrole-biotin): a new polymer for biomolecule grafting on electrode surfaces [J].
Cosnier, S ;
Lepellec, A .
ELECTROCHIMICA ACTA, 1999, 44 (11) :1833-1836
[9]   Elements of biosensor construction [J].
Davis, J ;
Vaughan, DH ;
Cardosi, MF .
ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (12) :1030-1035
[10]  
Ekins R, 1997, J Int Fed Clin Chem, V9, P100