Real-time PCR for detection and differentiation of gram-positive and gram-negative bacteria

被引：58

作者：

Klaschik, S ^{[1
]}

Lehmann, LE ^{[1
]}

Raadts, A ^{[1
]}

Book, M ^{[1
]}

Hoeft, A ^{[1
]}

Stuber, F ^{[1
]}

机构：

[1] Univ Bonn, Dept Anesthesia & Intens Care Med, D-53105 Bonn, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 11期

关键词：

D O I：

10.1128/JCM.40.11.4304-4307.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probes. All tested bacteria were identified correctly, and none gave a false-positive signal with the opposite Gram probe.

引用

页码：4304 / 4307

页数：4

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