Rapid methylation of cell proteins and lipids in Trypanosoma brucei

The fate of the [methyl-C-14] group of S-adenosylmethionine (AdoMet) in bloodstream forms of Trypanosoma brucei brucei, was studied. Trypanosomes were incubated with either [methyl-C-14]methionine, [U-C-14]methionine, S-[methyl-C-14]AdoMet or [S-35]methionine and incorporation into the total TCA precipitable fractions was followed. Incorporation of label into protein through methylation was estimated by comparing molar incorporation of [methyl-C-14] and [U-C-14]methionine to [S-35]methionine. After 4-h incubation with [U-C-14]methionine, [methyl-C-14]methionine or [S-35]methionine, cells incorporated label at mean rates of 2,880 pmol, 1,305 pmol and 296 pmol per mg total cellular protein, respectively. Cells incubated with [U-C-14] or [methyl-C-14]methionine in the presence of cycloheximide (50 mu g/ml) for four hours incorporated label eight- and twofold more rapidly, respectively, than cells incubated with [S-35]methionine and cycloheximide. [Methyl-C-14] and [U-C-14]methionine incorporation were > 85% decreased by co-incubation with unlabeled AdoMet (1 mM). The level of protein methylation remaining after 4-h treatment with cycloheximide was also inhibited with unlabeled AdoMet. The acid precipitable label from [U-C-14]methionine incorporation was not appreciably hydrolyzed by DNAse or RNAse treatment but was 95% solubilized by proteinase K. [U-C-14]methionine incorporated into the TCA precipitable fraction was susceptible to alkaline berate treatment, indicating that much of this label (55%) was incorporated as carboxymethyl groups. The rate of total lipid methylation was found to be 1.5 times that of protein methylation by incubating cells with [U-C-14]methionine for six hours and differential extraction of the TCA lysate. These studies show T. b. brucei maintains rapid lipid and protein methylation, confirming previous studies demonstrating rapid conversion of methionine to AdoMet and subsequent production of post-methylation products of AdoMet in African trypanosomes.