Evidence for activator and repressor functions of the response regulator MtrA from Corynebacterium glutamicum

被引:25
作者
Brocker, Melanie [1 ]
Bott, Michael [1 ]
机构
[1] Forschungszentrum Julich, Inst Biotechnol 1, D-52425 Julich, Germany
关键词
Corynebacterium glutamicum; two-component system; sensor kinase; response regulator; MtrA; MtrB;
D O I
10.1111/j.1574-6968.2006.00456.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Previous analysis of a Corynebacterium glutamicum Delta mtrAB mutant showed that the MtrAB two-component signal transduction system influences the expression of genes involved in cell wall metabolism or osmoregulation, but it remained unknown whether this influence is direct or indirect. In order to identify the direct target genes of the response regulator MtrA, chromatin immunoprecipitation as a genome-wide approach and DNA affinity chromatography as a gene-specific approach were used. The results indicate that mepA and nlpC, both encoding putative cell wall peptidases, are directly repressed by MtrA, whereas proP and betP, both encoding carriers for compatible solutes, are directly activated by MtrA.
引用
收藏
页码:205 / 212
页数:8
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