Cleavage of 16S rRNA within the ribosome by mRNA modified in the A-site codon with phenanthroline-Cu(II)

被引：10

作者：

Bucklin, DJ ^{[1
]}

vanWaes, MA ^{[1
]}

Bullard, JM ^{[1
]}

Hill, WE ^{[1
]}

机构：

[1] UNIV MONTANA, DIV BIOL SCI, MISSOULA, MT 59812 USA

来源：

BIOCHEMISTRY | 1997年 / 36卷 / 26期

关键词：

D O I：

10.1021/bi9624954

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cleavage of 16S rRNA was obtained through mRNA modified at position +5 with the chemical cleavage agent 1,10-o-phenanthroline. In the presence of Cu2+, and after addition of reducing agent to the modified mRNA-70S complex, cleavage of proximal nucleotides within the 16S rRNA occurred. Primer extension analysis of 16S rRNA fragments revealed that nucleotides 528-532, 1196, and 1396-1397 were cleaved. Nucleotides 1053-1055 were also cleaved bat did not show the same level of specificity as the former. These results provide evidence that at some point in the translation process these regions are all within 15 Angstrom of position +5, the A-site codon, on the mRNA.

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页码：7951 / 7957

页数：7

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