Conditional RNA interference achieved by Oct-1 POU/rtTA fusion protein activator and a modified TRE-mouse U6 promoter

被引：11

作者：

Fei, Zhaoliang

Chen, Zheng

Wang, Zhugang

Fei, Jian

机构：

[1] Chinese Acad Sci, SIBS, Model Organism Res Ctr,Lab Mol Cell Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China

[2] Shanghai NanFang Model Organism Res Ctr, Shanghai, Peoples R China

[3] Shanghai Med Univ 2, E Inst Shangai Univ, Model Organism Div, Shanghai, Peoples R China

[4] Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 354卷 / 04期

关键词：

RNA interference; Oct-1; tetracycline; conditional inducible; gene expression;

D O I：

10.1016/j.bbrc.2007.01.081

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

RNA interference (RNAi) is a powerful technique and is widely used to down-regulate expression of specific genes in cultured cells and in vivo. In this paper, we report our development of a new tetracycline-inducible RNAi expression using a modified TRE-mouse U6 promoter in which the distal sequence element (DSE) was replaced by the tetracycline-responsive element (TRE). The modified TRE-mouse U6 promoter can be activated by a Tet-on version tetracycline-regulated artificial activator rTetOct which was constructed by fusing the rtTA DNA binding domain with the Oct-1 POU activation domain. This rTetOct/TRE-U6 system was successfully applied to conditionally and reversibly down-regulate the expression of endogenous p53 gene in MCF7 cells, and the expression of P-defensin gene (mBin1b) either transiently expressed in COS7 cells or stably expressed in CHO cells. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：906 / 912

页数：7

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