Three-Dimensional Culture with Stiff Microstructures Increases Proliferation and Slows Osteogenic Differentiation of Human Mesenchymal Stem Cells

被引：29

作者：

Collins, John M. ^{[1
,2
]}

Ayala, Perla ^{[3
]}

Desai, Tejal A. ^{[3
]}

Russell, Brenda ^{[1
,2
]}

机构：

[1] Univ Illinois, Dept Physiol & Biophys, Coll Med, Chicago, IL 60612 USA

[2] Univ Illinois, Dept Bioengn, Coll Med, Chicago, IL 60612 USA

[3] Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA

来源：

SMALL | 2010年 / 6卷 / 03期

关键词：

cell lineage; gene expression; microenvironments; regenerative; medicine; BONE-MARROW; ADHESION; MATRIX; MIGRATION; RIGIDITY; 3D; SUBSTRATE; DIMENSION; NICHE; SHAPE;

D O I：

10.1002/smll.200901757

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

(Figure Presented) A small quantity of stiff microstructures in a 3D matrix regulates human mesenchymal stem cells to increase proliferation and slow differentiation after 10 days in culture. Cell morphology, clustering, and gene expression are significantly different although the stiff microstructures are only 0.07% of the total gel volume. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：355 / 360

页数：6

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