Developing arbovirus resistance in mosquitoes

被引：50

作者：

Olson, KE ^{[1
]}

Adelman, ZN ^{[1
]}

Travanty, EA ^{[1
]}

Sanchez-Vargas, I ^{[1
]}

Beaty, BJ ^{[1
]}

Blair, CD ^{[1
]}

机构：

[1] Colorado State Univ, Dept Microbiol, Arthropod Borne & Infect Dis Lab, Ft Collins, CO 80523 USA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2002年 / 32卷 / 10期

关键词：

dengue; Aedes aegypti; mosquito; genetic manipulation; transformation; transposable element; pathogen-derived resistance; RNA interference; post-transcriptional gene silencing;

D O I：

10.1016/S0965-1748(02)00096-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diseases caused by arthropod-borne viruses are increasingly significant public health problems, and novel methods are needed to control pathogen transmission. The hypothesis underlying the research described here is that genetic manipulation of Aedes aegypti mosquitoes can profoundly and permanently reduce their competence to transmit dengue viruses to human hosts. Recent key findings now allow us to test the genetic control hypothesis. We have identified viral genome-derived RNA segments that can be expressed in mosquito midguts and salivary glands to ablate homologous virus replication and transmission. We have demonstrated that both transient and heritable expression of virus-derived effector RNAs in cultured mosquito cells can silence virus replication, and have characterized the mechanism of RNA-mediated resistance. We are now developing virus-resistant mosquito lines by transformation with transposable elements that express effector RNAs from mosquito-active promoters. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1333 / 1343

页数：11

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