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Ethylene promotes submergence-induced expression of OsABA8ox1, a gene that encodes ABA 8′-hydroxylase in rice
被引:213
作者:
Saika, Hiroaki
Okamoto, Masanori
Miyoshi, Kentaro
Kushiro, Tetsuo
Shinoda, Shoko
Jikumaru, Yusuke
Fujimoto, Masaru
Arikawa, Taku
Takahashi, Hirokazu
Ando, Miho
Arimura, Shin-ichi
Miyao, Akio
Hirochika, Hirohiko
Kamiya, Yuji
Tsutsumi, Nobuhiro
Nambara, Eiji
Nakazono, Mikio
机构:
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Plant Mol Genet, Tokyo 1138657, Japan
[2] RIKEN, Plant Sci Ctr, Growth Regulat Res Grp, Kanagawa 2300045, Japan
[3] Tokyo Metropolitan Univ, Dept Biol Sci, Tokyo 1920397, Japan
[4] Natl Inst Argobiol Sci, Tsukuba, Ibaraki 3058602, Japan
关键词:
ABA 8 '-hydroxylase;
ethylene;
rice;
submergence;
D O I:
10.1093/pcp/pcm003
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
A rapid decrease of the plant hormone ABA under submergence is thought to be a prerequisite for the enhanced elongation of submerged shoots of rice (Oryza sativa L.). Here, we report that the level of phaseic acid (PA), an oxidized form of ABA, increased with decreasing ABA level during submergence. The oxidation of ABA to PA is catalyzed by ABA 8'-hydroxylase, which is possibly encoded by three genes (OsABA8ox1, -2 and -3) in rice. The ABA 8'-hydroxylase activity was confirmed in microsomes from yeast expressing OsABA8ox1. OsABA8ox1-green fluorescent protein (GFP) fusion protein in onion cells was localized to the endoplasmic reticulum. The mRNA level of OsABA8ox1, but not the mRNA levels of other OsABA8ox genes, increased dramatically within 1 h after submergence. On the other hand, the mRNA levels of genes involved in ABA biosynthesis (OsZEP and OsNCEDs) decreased after 1-2 h of submergence. Treatment of aerobic seedlings with ethylene and its precursor, 1-aminocyclopropane-1-carboxylate (ACC), rapidly induced the expression of OsABA8ox1, but the ethylene treatment did not strongly affect the expression of ABA biosynthetic genes. Moreover, pre-treatment with 1-methylcyclopropene (1-MCP), a potent inhibitor of ethylene action, partially suppressed induction of OsABA8ox1 expression under submergence. The ABA level was found to be negatively correlated with OsABA8ox1 expression under ACC or 1-MCP treatment. Together, these results indicate that the rapid decrease in ABA levels in submerged rice shoots is controlled partly by ethylene-induced expression of OsABA8ox1 and partly by ethylene-independent suppression of genes involved in the biosynthesis of ABA.
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页码:287 / 298
页数:12
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