Identification of GFR α-2 isoforms in myenteric plexus of postnatal and adult rat intestine

被引：11

作者：

Dolatshad, NF ^{[1
]}

Silva, AT ^{[1
]}

Saffrey, MJ ^{[1
]}

机构：

[1] Open Univ, Dept Biol Sci, Milton Keynes MK7 6AA, Bucks, England

来源：

MOLECULAR BRAIN RESEARCH | 2002年 / 107卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

neurturin; glial cell line-derived neurotrophic factor; c-Ret enteric nervous system; myenteric plexus;

D O I：

10.1016/S0169-328X(02)00446-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Glial cell line-derived neurotrophic factor family receptor alpha-2 (GFR alpha-2) is a GPI-linked receptor that preferentially binds neurturin (NTN), a member of the glial cell line-derived neurotrophic factor (GDNF) family. Three splice isoforms of GFR alpha-2 have been identified previously in mouse tissues, but the occurrence of splice isoforms in rats has not been described. The aim of this study was therefore to identify GFR alpha-2 splice isoforms in rat tissues using reverse transcription-polymerase chain reaction (RT-PCR) and gene cloning. Three isoforms were identified and sequenced, and named GFR alpha-2(a), (b) and (c), according to the nomenclature used for the previously identified mouse isoforms. The GFR alpha-2(a) and (b) isoforms were identical to those previously described in mice. The GFR alpha-2(c) isoform was novel. Sequences for GFR alpha-2(b) and (c) were deposited in the GenBank database (accession numbers GI: 16797788 and 16797786, respectively). All three isoforms were expressed in the brain, kidney, and intestine of both postnatal and adult rats. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：32 / 38

页数：7

共 29 条

[1] GDNF family neurotrophic factor signaling: Four masters, one servant? [J].

Airaksinen, MS ;

Titievsky, A ;

Saarma, M .

MOLECULAR AND CELLULAR NEUROSCIENCE, 1999, 13 (05) :313-325

[2] The GDNF family ligands and receptors - implications for neural development [J].

Baloh, RH ;

Enomoto, H ;

Johnson, EM ;

Milbrandt, J .

CURRENT OPINION IN NEUROBIOLOGY, 2000, 10 (01) :103-110

[3] GFRα3 is an orphan member of the GDNF/neurturin/persephin receptor family [J].

Baloh, RH ;

Gorodinsky, A ;

Golden, JP ;

Tansey, MG ;

Keck, CL ;

Popescu, NC ;

Johnson, EM ;

Milbrandt, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (10) :5801-5806

[4] TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret [J].

Baloh, RH ;

Tansey, MG ;

Golden, JP ;

Creedon, DJ ;

Heuckeroth, RO ;

Keck, CL ;

Zimonjic, DB ;

Popescu, NC ;

Johnson, EM ;

Milbrandt, J .

NEURON, 1997, 18 (05) :793-802

[5] Artemin, a novel member of the GDNF ligand family, supports peripheral and central neurons and signals through the GFRα3-RET receptor complex [J].

Baloh, RH ;

Tansey, MG ;

Lampe, PA ;

Fahrner, TJ ;

Enomoto, H ;

Simburger, KS ;

Leitner, ML ;

Araki, T ;

Johnson, EM ;

Milbrandt, J .

NEURON, 1998, 21 (06) :1291-1302

[6] GFRα1 is an essential receptor component for GDNF in the developing nervous system and kidney [J].

Cacalano, G ;

Fariñas, I ;

Wang, LC ;

Hagler, K ;

Forgie, A ;

Moore, M ;

Armanini, M ;

Phillips, H ;

Ryan, AM ;

Reichardt, LF ;

Hynes, M ;

Davies, A ;

Rosenthal, A .

NEURON, 1998, 21 (01) :53-62

[7] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[8] Restricted diet rescues rat enteric motor neurones from age related cell death [J].

Cowen, T ;

Johnson, RJR ;

Soubeyre, V ;

Santer, RM .

GUT, 2000, 47 (05) :653-660

[9]

Davies AM, 1997, NEURON, V19, P485

[10] Cloning of a novel murine isoform of the glial cell line-derived neurotrophic factor receptor [J].

Dey, BK ;

Wong, YW ;

Too, HP .

NEUROREPORT, 1998, 9 (01) :37-42

← 1 2 3 →