Development of a recombinant nucleoprotein-based enzyme-linked immunosorbent assay for quantification of antibodies against porcine reproductive and respiratory syndrome virus

被引：10

作者：

Witte, SB

Chard-Bergstrom, C

Loughin, TA

Kapil, S

机构：

[1] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA

[2] Kansas State Univ, Coll Arts & Sci, Dept Stat, Manhattan, KS 66506 USA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2000年 / 7卷 / 04期

关键词：

D O I：

10.1128/CDLI.7.4.700-702.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.

引用

页码：700 / 702

页数：3

共 19 条

[1] CHARACTERIZATION OF SWINE INFERTILITY AND RESPIRATORY SYNDROME (SIRS) VIRUS (ISOLATE ATCC VR-2332) [J].