A protein fluorescence amplifier: Continuous fluorometric assay for Rab geranylgeranyltransferase

被引：22

作者：

Wu, Yao-Wen

Waldmann, Herbert

Reents, Reinhard

Ebetino, Frank H.

Goody, Roger S.

Alexandrova, Kirill

机构：

[1] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany

[2] Procter & Gamble Co, Hlth Care Res Ctr, Mason, OH 45040 USA

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 12期

关键词：

fluorescence spectroscopy; fluorescent probes; inhibitors; NBd; prenylation; RabGGTase;

D O I：

10.1002/cbic.200600377

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 23-fold enhancement of fluorescence is observed upon RabGGTase-mediated protein prenylation by NBD-FPP. We propose that the chaperone of prenylated Rab GTPases, REP, which harbors the conjugated prenyl moieties, functions as a fluorescence amplifier and leads to intermolecular fluorescence enhancement. This reaction was characterized and used to develop a fluorescent prenylation assay that can be adapted for a high-throughput format. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：1859 / 1861

页数：3

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