From genome to proteome: Protein map of Haemophilus influenzae

被引：42

作者：

Langen, H ^{[1
]}

Gray, C ^{[1
]}

Roder, D ^{[1
]}

Juranville, JF ^{[1
]}

Takacs, B ^{[1
]}

Fountoulakis, M ^{[1
]}

机构：

[1] F HOFFMANN LA ROCHE & CO LTD, PHARMACEUT RES, INFECT DIS, CH-4070 BASEL, SWITZERLAND

来源：

ELECTROPHORESIS | 1997年 / 18卷 / 07期

关键词：

amino acid analysis; mass spectrometry; protein identification; two-dimensional polyacrylamide gel electrophoresis;

D O I：

10.1002/elps.1150180727

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-resolution two-dimensional (2-D) polyacrylamide gel electrophoresis allows the separation of complex biological mixtures (i.e., several hundred proteins from a bacterial cell lysate) in a single experiment. In this report proteins from Haemophilus influenzae were separated by 2-D gels and analyzed by peptide mass fingerprinting and/or amino acid analysis. By comparing the peptide mass profiles and the amino acid composition with the Haemophilus influenzae database, 119 protein spats were identified. The combination of amino acid analysis and peptide mass fingerprinting is a powerful tool for a rapid and economical identification of a large number of proteins resolved by 2-D gels. Studies on gene regulation and changes of protein expression upon drug treatment require quick and serial analysis techniques to efficiently identify potential new drug targets.

引用

页码：1184 / 1192

页数：9

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