Encapsidation of potyviral RNA in various forms of transgene coat protein is not correlated with resistance in transgenic plants

被引：13

作者：

Hammond, J

Dienelt, MM

机构：

[1] U. States Department of Agriculture, Agricultural Research Service, Floral and Nursery Plants Res. Unit, Beltsville

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1997年 / 10卷 / 08期

关键词：

aphid transmissibility; phenotypic mixing;

D O I：

10.1094/MPMI.1997.10.8.1023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transgenic plants expressing either bean yellow mosaic potyvirus or chimeric potyvirus coat protein (CP) were inoculated with various potyviruses. Antigen-coated plate, indirect enzyme-linked immunosorbent assay and immunoelectron microscopy of virus purified from transgenic plants showed that progeny virions contained from <1% to as much as 25% transgenic CP. Different levels of transcapsidation may reflect the extent of compatibility between transgene CP and the viral CP.

引用

页码：1023 / 1027

页数：5

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