Comparison of Database Search Strategies for High Precursor Mass Accuracy MS/MS Data

被引：106

作者：

Hsieh, Edward J. ^{[1
]}

Hoopmann, Michael R. ^{[1
]}

MacLean, Brendan ^{[1
]}

MacCoss, Michael J. ^{[1
]}

机构：

[1] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2010年 / 9卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Proteomics; Database search; Precursor estimation; Mass accuracy; PEPTIDE IDENTIFICATION; SPECTROMETRIC DATA; TANDEM; SPECTRA; REDUCTION; ALGORITHM; PROTEINS;

D O I：

10.1021/pr900816a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In shotgun proteomics, the analysis of tandem mass spectrometry data from peptides can benefit greatly from high mass accuracy measurements. In this study, we have evaluated two database search strategies which use high mass accuracy measurements of the peptide precursor ion. Our results indicate that peptide identifications are improved when spectra are searched with a wide mass tolerance window and precursor mass is used as a filter to discard incorrect matches. Database searches with a peptide data set constrained to peptides within a narrow mass window resulted in fewer peptide identifications but a significantly faster database search time.

引用

页码：1138 / 1143

页数：6

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