Identification of a Novel Astrovirus (Astrovirus VA1) Associated with an Outbreak of Acute Gastroenteritis

被引：158

作者：

Finkbeiner, Stacy R. ^{[1
,2
]}

Li, Yan ^{[3
]}

Ruone, Susan ^{[3
]}

Conrardy, Christina ^{[3
]}

Gregoricus, Nicole ^{[3
]}

Toney, Denise ^{[4
]}

Virgin, Herbert W. ^{[1
,2
]}

Anderson, Larry J. ^{[3
]}

Vinje, Jan ^{[3
]}

Wang, David ^{[1
,2
]}

Tong, Suxiang ^{[3
]}

机构：

[1] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

[3] Ctr Dis Control & Prevent, Div Viral Dis, Atlanta, GA 30333 USA

[4] Consolidated Lab Serv, Commonwealth Virginia Div, Richmond, VA 23219 USA

来源：

JOURNAL OF VIROLOGY | 2009年 / 83卷 / 20期

基金：

美国国家卫生研究院;

关键词：

CHILDREN; DIARRHEA; NOROVIRUS; SEQUENCE; GENOME; VIRUS;

D O I：

10.1128/JVI.00998-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The etiology of a large proportion of gastrointestinal illness is unknown. In this study, random Sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. Multiple sequences with limited identity to known astroviruses were identified. Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends generated a complete genome of 6,586 nucleotides. Phylogenetic analysis demonstrated that this virus, named astrovirus VA1 (AstV-VA1), is highly divergent from all previously described astroviruses. Based on RT-PCR, specimens from multiple patients in this outbreak were unequivocally positive for Ast-VA1.

引用

页码：10836 / 10839

页数：4

共 22 条

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