Mass transport limitations reduce the effective stereospecificity in enzyme-catalyzed kinetic resolution

被引：37

作者：

Rotticci, D

Norin, T

Hult, K ^{[1
]}

机构：

[1] Royal Inst Technol, Dept Biotechnol, SE-10044 Stockholm, Sweden

[2] Royal Inst Technol, Dept Chem, SE-10044 Stockholm, Sweden

来源：

ORGANIC LETTERS | 2000年 / 2卷 / 10期

关键词：

D O I：

10.1021/ol005639m

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The kinetic resolution of seudenol catalyzed by Candida antarctica lipase a in hexane was investigated. Large differences in reaction rate and stereospecificity were observed when different enzyme preparations were used, These differences were ascribed to mass transport limitations which reduced both reaction rate and stereospecificity. Lyophilized enzyme preparations were more apt to give this problem than immobilized preparations. Further, low substrate concentrations enhanced the effect. Thus, high alcohol concentrations and enzyme immobilization can be recommended.

引用

页码：1373 / 1376

页数：4

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