Functional expression of heterologous UDP-galactose-4-epimerase in Agrobacterium sp via a broad host-range expression vector

被引：2

作者：

He, DS ^{[1
]}

Chen, RZR ^{[1
]}

机构：

[1] Virginia Commonwealth Univ, Dept Chem Engn, Richmond, VA 23284 USA

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 19期

关键词：

Agrobacterium sp; broad-host-range expression vector; epimerase; heterologous protein expression;

D O I：

10.1023/A:1020390410512

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report here the first functional expression of a heterologous protein in an Agrobacterium sp. strain (LTU261). A 1014 bp gene coding for the dimeric 79 kDa UDP-galactose-4-epimerase from E. coli was successfully cloned into a 11 kb broad host-range expression vector. Both expression level and activity level in Agrobacterium sp. LTU 261 were about one-tenth of the level obtained in E. coli from the same plasmid. The success of the heterologous expression in Agrobacterium sp. opens up possibilities for introducing new products into this organism and offers opportunities for improvement in production and modification of the existing bioproducts from this organism.

引用

页码：1599 / 1603

页数：5

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