Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm

被引：34

作者：

Chereguini, O ^{[1
]}

Cal, RM ^{[1
]}

Dreanno, C ^{[1
]}

deBaulny, BO ^{[1
]}

Suquet, M ^{[1
]}

Maisse, G ^{[1
]}

机构：

[1] CTR OCEANOG SANTANDER,IEO,SANTANDER 39080,SPAIN

来源：

AQUATIC LIVING RESOURCES | 1997年 / 10卷 / 04期

关键词：

turbot; sperm; storage; cryopreservation;

D O I：

10.1051/alr:1997028

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 degrees C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.

引用

页码：251 / 255

页数：5

共 18 条

[1] SHORT-TERM PRESERVATION OF SPERM UNDER OXYGEN ATMOSPHERE IN RAINBOW-TROUT (SALMO-GAIRDNERI) [J].